- 99 Overall Club: QB Peyton Manning (Colts), RB LaDainian Tomlinson (Chargers), MLB Brian Urlacher (Bears), FS Ed Reed (Ravens), CB Champ Bailey (Broncos)
- QB Trent Green was originally listed on the Kansas City Chiefs roster, but he was traded to the Miami Dolphins shortly before the game released, so he is still listed on the Kansas City Chiefs roster below.
- Kansas City Chiefs QB Trent Green signed with the Miami Dolphins roster in Roster Update #1
- Miami Dolphins QB Daunte Culpepper signed with the Oakland Raiders roster in Roster Update #1.
- Atlanta Falcons QB Michael Vick was removed from the game in Roster Update #2.
- Cincinnati Bengals sign QB Ryan Fitzpatrick in Roster Update #2.
- Jacksonville Jaguars release QB Byron Leftwich in Roster Update #2.
- Atlanta Falcons sign QB Byron Leftwich in Roster Update #3.
- Carolina Panthers sign QB Matt Moore in Roster Update #4.